Free Pearson Edexcel International A-level Biology · Unit 6

Spirometer traces and exercise comparisons

What you’ll learn

  • Calculate breathing rate, tidal volume and minute ventilation.
  • Interpret oxygen-consumption trends from a stated trace model.
  • Evaluate repeated measurements and participant-related variation.

Understand the idea

Core practical 17 uses spirometer traces to study exercise-related changes. Tidal volume is the volume moved in one normal breath, measured from the peak-to-trough difference for a complete breathing cycle. Breathing frequency is complete cycles per minute. Respiratory minute ventilation is tidal volume multiplied by breathing frequency, so a change in either factor can alter the total air moved each minute.

In a specified closed-system record where carbon dioxide is absorbed, a gradual fall in the gas-volume baseline can be used to estimate oxygen uptake. Compare like points in the breathing cycle or an appropriate mean trend over a sufficient interval. Do not confuse the oscillation amplitude with the baseline gradient, and check the time-axis units before converting to a per-minute rate. Leaks and temperature changes can invalidate a simple oxygen-consumption interpretation.

Comparing the same participant before and after an agreed activity reduces some between-person variation, but order, recovery time and previous activity still matter. Several participants are needed for a broader conclusion, with privacy, consent and the right to stop respected. These questions use supplied traces; any actual human exercise or breathing-apparatus activity requires appropriate supervision, hygiene and participant suitability checks.

Key formulae

f=complete breathstime in minutesf=\frac{\mathrm{complete\ breaths}}{\mathrm{time\ in\ minutes}}

V˙E=VTf\dot V_E=V_Tf

rO2=baseline volume fallΔtunder the stated trace conditionsr_{\mathrm{O_2}}=\frac{\mathrm{baseline\ volume\ fall}}{\Delta t}\quad\text{under the stated trace conditions}

Read tidal volume from peak to trough, not from amplitude

Conditions. Idealised trace V = 0.5 + 0.25 cos(2πt/5). No baseline drift; illustrative breath period 5 s and oscillation amplitude 0.25 dm³. This is a synthetic educational trace, not a diagnostic record.

Time / s; Relative gas volume / dm³.

Peak-to-trough tidal volume is 0.50 dm³, twice the amplitude. Four complete breaths in 20 s give 12 breaths min⁻¹ and minute ventilation 6.0 dm³ min⁻¹.

Open the interactive lesson to view plotted curves and coordinate tables.

Worked example

A supplied trace shows 10 complete breaths in 30 s30\ \mathrm{s}, each with a tidal volume of 0.80 dm30.80\ \mathrm{dm^3}. In the stated closed-system model with carbon dioxide absorbed, the mean gas-volume baseline falls by 0.60 dm30.60\ \mathrm{dm^3} over 120 s120\ \mathrm{s}. Calculate minute ventilation and oxygen uptake per minute.

  1. Ten breaths in half a minute gives 20 breaths min−120\ \mathrm{breaths\,min^{-1}}.
  2. Minute ventilation is 0.80×20=16 dm3 min−10.80\times20=16\ \mathrm{dm^3\,min^{-1}}.
  3. The baseline fall occurs over 2.0 min2.0\ \mathrm{min}, so oxygen uptake is 0.60/2.0=0.30 dm3 min−10.60/2.0=0.30\ \mathrm{dm^3\,min^{-1}} under the stated model.

Answer: Minute ventilation 16 dm3 min−116\ \mathrm{dm^3\,min^{-1}}; oxygen uptake 0.30 dm3 min−10.30\ \mathrm{dm^3\,min^{-1}}.

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